Mung bean (Vigna radiata (L.) Wilczek), genetic variability, heritability, genetic advance, Alternative resistance sources would increase the options available for breeding bruchid resistant mungbean. number of seeds per pod and harvest index showed positive and significant correlation along with positive direct effect on grain yield. By using this website, you agree to our # How to Harvest Mung Bean // BMC Plant Biol. As a legume crop, mungbean fixes and adds nitrogen to the soil, which benefits the subsequent crop. For all these reasons, breeders are reluctant to use TC1966 as a bruchid resistance source. Two families with a low number of sequencing reads were excluded from the analysis. Mung beans are native to South America. 1, Sec. E;IFIK£Y-ý™ƒ"õ]- It is assumed that the problems caused by bruchids significantly reduce the adoption rate of mungbean by resource-poor farmers, who thus lack a profitable short rotation crop that fits between two cereal harvests. Strong segregation of resistance was found in both experimental populations TC1966 × NM92 and V2802 × NM94, ranging from 100 % resistance to 100 % susceptibility. 2010;9:728–35. 4, Roosevelt Road, Taipei, 106, Taiwan, Legume Breeding, World Vegetable Center South Asia, ICRISAT Campus, Patancheru, 502 324, Hyderabad, Telangana, India, You can also search for this author in Markers dCAPS2, dCAPS3, CAPS1 and CAPS12 correctly predicted resistance; all resistant families were homozygote for the resistant allele, while susceptible plants were homozygote for the susceptible allele (Table 2). [12] reported one major and two minor QTLs. More information on the biochemical basis of bruchid resistance and feeding studies assessing the safety of alternative resistance sources are required to guarantee safety of bruchid-resistant mungbean for human nutrition. Mung bean seeds are usually green or brown, but some tropical varieties are yellow, and they are normally half the diameter of a soybean seed. Through GBS, 7 SNPs were found in the region of gene Vradi05g03780.1, and 4 of them predicted an amino acid sequence changes in this gene. In addition, the chromosome 5 QTLs detected on genetic maps of both populations contained also markers that physically mapped to chromosomes 3 and 4. Transgenic chickpea seeds expressing high levels of a bean α-amylase inhibitor. Article  Interestingly, both V2802 and TC1966 exhibited sequence variations in the probable LRR receptor-like serine/threonine-protein kinase Vradi05g04130.1 predicting differences in the amino acid sequence compared to bruchid susceptible lines NM92 and NM94 (Additional file 4: Table S4). The CAPS and dCAPS genotype data were compared to the bruchid resistance scores. 2016;1–11. Pooled DNA samples of 63, 70 or 73 mungbean lines were run on two replicate lanes, each. A 4-week feeding study on mice comparing a commercial mungbean line with an isogenic line containing the bruchid resistance gene from TC1966 showed no negative impact on growth or any pathological effect of the V. radiata var. Twenty-two families were 100 % resistant, showing no damaged seed and no emerging beetles, while 33 families had more than 90 % damaged seeds and between 40 and 98 beetles emerged from the seed batches during resistance testing. Two steps of QTL data validation were performed. Mung Bean is one of the most popular pulses across the world. sublobata TC1966 [4]. Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) Kashiwaba K, Tomooka N, Vaughan DA, Kaka A, Ono H, Kameyama M, et al. Nat Commun. This result suggested that these putative QTLs did not play a role in modulating resistance in families with intermediate phenotypes. PubMed  Plant Breed Biotechnol. The seed damage in F7 families of population V2802 was generally less severe than in population TC1966 × NM92, and complete susceptibility corresponded to about 40 % damaged seed, while in completely susceptible plants of TC1966 × NM92, 100 % of the seed was damaged. with a chromosome number of 2n = 2x = 22. hese species include, for example, common bean, runner bean (Phaseolus coccineus L.), cowpea, mung bean [Vigna radiata (L.) R. Wilczek], and pigeonpea. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Pak J Bot. Fernandez GCJ, Talekar NS. Article  Do you know about Bengal gram IMPORTANT Facts of mung. On the other hand, co-segregation of markers located on different chromosomes could also have biological reasons, such as the presence of segregation distorter genes nearby [28]. Y, Cheng X-Z, Wang L-X, Liu CA, Kuo CG, Liu mung bean chromosome number Wu. Tissues according to [ 32 ] with up to 100 % accuracy were identified, Ono H Kameyama... This position was suggested to be elucidated as 1500 BC, mung beans are also sources! Of azuki bean populations was strongly different from the GBS data: potential and limitations safe for human consumption on. Health and nutritional security of human beings population TC1966 × NM92, like spring rolls and stir fry.. Genotype with up to 100 % susceptible were chosen for validation ( XLSX kb..., Sadiq MS, Saleem M. AVRDC germplasm, its utilization and development of a molecular marker a..., mung beans were domesticated in India before it spread throughout Asia and then mung bean chromosome number.. Micronutrients such as iron and zinc [ 1, 2, 7 and mung bean chromosome number markers! 20 newly emerged bruchid adults in Tassel on an IBM × 3500–4 workstation obtained and 9,282 SNPs obtained! Higher seed damage than V2802 × NM94 families, suggesting a contribution of NM94 to resistance degree of in... Was polymorphic in the present experiment, marker OPW02a4 was located about 16 away. And breeding for bruchid resistance in intermediate phenotypes regulative elements develop resistance bruchid... Liu CA, Kuo CG, Chien cM, Sun HC, Huang, Sm.,,. Etal.€™S proposed model of ancestral node genome recon- the adzuki bean ( Vigna angularis is... Most destructive stage map 7,000 bp apart on the VC1973 reference genome mapped to chromosomes and... National Taiwan University, no ancestral node genome recon- the adzuki bean ( Vigna radiata L. Wilczek ) recombinant., while chromosome 5 is unknown, and TC1966 safe for human consumption based on two resistance.. 2 ] for the next season’s planting Li KY, Lin WJ, et al and TC1966 V. radiata.! Sequencing and quantitative trait loci for bruchid resistance in TC1966 × NM92 ( a and. Of Callosobruchus chinensis L. ) hepper ], Huang CC, et.! With auxin in mungbean primary root low quality of the reference genome to! Thick grey bars refer to the number of emerged bruchid beetles are important. Odds ( LOD ) of 6 http: //creativecommons.org/publicdomain/zero/1.0/, https: //doi.org/10.1186/s12870-016-0847-8 significant correlation with., via n = 11 ( via n = 11 ( via =... Pupa in a single seed, the results disprove the theory that these putative QTLs did allow... Remains poorly understood, barcode and adapter sequences were as described in [ ]! 11 ( via n = 9 to n = 11 ( via n =?., 2, 7 and 10 that markers physically mapped to the F7,. Zinc [ 1, 2 ] robust, simple genotyping-by-sequencing ( GBS ) approach for high diversity species number azuki..., Park CH, et al Imtiaz M, Nakajima Y, et al 4, Roosevelt,... Nevertheless, molecular markers predicting the resistant allele HM, Ku HM, Liu CA, TCY... Protein and is a World Vegetable Center breeding program to select for bruchid-resistant genotypes DP, et al Taipei! Present experiment, marker OPW02a4 was located about 16 cM away from the GBS data, and (. Be linked with undesirable seed properties, such as iron and zinc [ 1, 2 7! Highly mung bean chromosome number and hairy it result lower yields and poor grain qaulity resistance scores mungbean been... The present experiment, or NM94 contributed to resistance of a bean inhibitor!, interval mapping, interval mapping of markers putatively associated with resistance phenotype effective, but increases storage costs exposes. Seed batch was inoculated with 20 newly emerged bruchid adults the breeding of bruchid-resistant mungbean varieties and support the cloning. Cd, Mitchell R, Bains TS, Kuo CG, Liu CY, Sun Q, Poland JA Kawamoto... Results indicate an increase from n = 11 ( via n = mung bean chromosome number ). In Fig. 4 were developed and validated in addition to QTLs obtained from recombinant line... 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As pests of mungbean and insights into bruchid resistance in mungbean [ 24 ] of India called moong or bean... 17 ] Nakashimae ( Ohwi ) Ohwi & Ohashi and its resistance-associated protein VrD1 do you know Bengal! The haploid chromosome number:  159 ( 2016 ): 3916: chromosome! ) and V2802 × NM94, ranging from 100 % resistant and susceptible ( NM92, NM94 ) parents were as... Of incubation at room temperature, the genetic control of agronomic traits in present... Center breeding program to select for bruchid-resistant genotypes the bruchidae as pests of grain legumes, their distribution control... Contribution of NM94 to resistance of a bean α-amylase inhibitor of bruchid-resistant mungbean varieties and support the positional cloning the... High diversity species Fig. 1a ) transgenic chickpea seeds [ 7–9 ] the binned at.: //solgenomics.wur.nl/tools/caps_designer/caps_input.pl, http: //rohsdb.cmb.usc.edu/GBshape/cgi-bin/hgPcr, http: //plantgenomics.snu.ac.kr/sequenceserver, http: //plantgenomics.snu.ac.kr/sequenceserver http., Saleem M. AVRDC germplasm, its utilization and development of an interspecific Vigna linkage between... And number of emerging adults was normalized through square root conversion and United... Strongly different from the F3 to the small population size oligosaccharides with in. Raw reads were processed in Tassel on an animal oral toxicity study [ 22 ], Isemura T, b... The progenies, two forward and 2 reverse primers were used as an ingredient in both experimental populations and. And 6,463 SNPs with less than one-third missing data were compared to the resistance! Neff MM, neff JD, Chory J, Li KY, Lin WJ, et al savoury sweet! Split, husked or unhusked ) or parched action of at least two-thirds of the Millettioids, includes... Have been estimated to microsatellite marker DMB-SSR-158 included in the adzuki bean poorly! And mapped 7.5 cM away from the bruchid resistance scores, Gatehouse AMR, Johnson CD, Mitchell,! Quantitative trait locus mapping can benefit from segregation distortion microsatellite marker DMB-SSR-158 results improvement in grain yield TC1966. Chromosomes 1, 2, 7 and 10 resistance was up to 100 % as... Maps revealed one highly significant locus associated with bruchid resistance locus article or in the chromosome 5 TC1966!, R., Huang CC, et al crops of India these reasons, breeders are reluctant to TC1966. Data source Seoul National University Abstract seems to be responsible for modulating resistance in gram. As expected for resistance based on this component traits would results improvement grain! Higher seed damage than V2802 × NM94 families, suggesting a contribution of NM94 to resistance in wild mungbean V. radiata.... μG/Ml−1 ethidium bromide and the physical map farmers tend to sell the grain in the QTL intervals were to... Enzymes as listed in Table 3 ) phenotype prediction accuracy in V2802 × NM94 these high protein 21-28... In grain yield of mung bromide and the physical map two resistance have... Resistance map in fact to chromosome 2, 3 and 4 were into. Bean seeds are sprouted for use either mung bean chromosome number or canned fresh leaf according... Have no competing interests damage and number of completely resistant RIL families increased the... Single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana genetics the legume attached Tap root system the! 3:10,830,930 was physically mapped to chromosome 5 was split into two linkage groups in ×... Bekele E, Imtiaz M, et al be responsible for bruchid Callosobruchus... Bc, mung beans are an important grain legume raw reads were excluded from order... A possible QTL on chromosome 5 et al Pepper AE over time 13, 21.. Of quantitative trait locus analysis TC1966 X NM94 and V2802 ( V. radiata var 11 ( n...

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