Due to their direct involvement in serious nosocomial infections, A. baumannii and P. aeruginosa are of great concern for public health [25]. Author information: (1)Research Associate, Department of Pedodontics and Preventive Dentistry, Maulana Azad Institute of Dental … and S.B. Scanning electron micrographs of MTX implants after bacterial contamination Group V. Low magnification showing the biofilm formation on implant threads: (A) 200×; and (B) 1000×. Significance was set at 5%. Albertini et al. Additionally, previous in vitro study showed that decontaminating the implant surface using an Er,Cr:YSGG laser favors osteoblast attachment [19]. Magnification: 12,000×. A dental laser is a type of laser designed specifically for use in oral surgery or dentistry.. The ePub format is best viewed in the iBooks reader. All implants were then divided into five groups for the decontamination procedure. Ušjak D., Ivković B., Božić D.D., Bošković L., Milenković M. Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. Koo K.-T., Khoury F., Keeve P.L., Schwarz F., Ramanauskaite A., Sculean A., Romanos G. Implant Surface Decontamination by Surgical Treatment of Periimplantitis. and P. aeruginosa were selected for this study. Magnification: 12,000×. For these purposes, Er,Cr:YSGG, 1 Er:YAG, 2 CO 2, 3 and Ga/Al/As diode 4,5 laser systems are available. On the contrary, higher-power lasers are expensive and can encourage an undesirable increase in temperature and alterations in the surface [14,15]. An Er,Cr:YSGG laser was used to ablate human dental hard tissues with the output powers of 3 to 6 W. Ablation extent with or without water spray at different output powers was measured, and the morphological changes on enamel and dentin were also investigated by stereoscopy and scanning electron microscopy (SEM). In October 1998, the US Food and Drug Administration (FDA) have approved the Er,Cr:YSGG laser for dental After decontamination, colony forming units (CFU) were counted and implants were prepared for SEM analysis. suggested that P. aeruginosa in epithelial tissues might be of potential use as a diagnostic tool for periodontitis [35]. Influence of an Erbium, Chromium-Doped Yttrium, Scandium, Gallium, and Garnet (Er,Cr:YSGG) Laser on the Reestablishment of the Biocompatibility of Contaminated Titanium Implant Surfaces. Colony forming units (CFU) for the tested bacteria in relation to the different decontamination method. and F.A.O. Strever et al. [33,34] reported higher proportions of Pseudomonas species in peri-implant sites [34]. Toma S., Behets C., Brecx M.C., Lasserre J.F. Other authors have also reported associations between these species and sites and/or subjects with periodontal disease, particularly in HIV-infected patients [29]. Detection of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival biofilm of HIV-infected subjects undergoing HAART with chronic periodontitis. Then laser irradiation was performed using a GaAlAs low-level diode laser (Lasercat 500, Medsolution, Radolfzell, Germany) at a wavelength of 650 nm and output power of 50 mW at a distance of 0.5 mm for 60 s per implant surface. Scanning electron micrographs of MTX implants used in the current study (A,B) Higher magnification of the MTX surface before surface contamination: (C) 5000×; and (D) 12,000×. Two reference strains, A. baumannii (ATCC BAA-1710) and P. aeruginosa (ATCC 27853), were grown on Luria–Bertani media (LB) at 37 °C and overnight cultures were used [20,26]. Normality was assessed using the Kolmogorov–Smirnov test. The effect of a decontamination protocol on contaminated titanium dental implant surfaces with different surface topography in edentulous patients. We are experimenting with display styles that make it easier to read articles in PMC. Schwarz F., Nuesry E., Bieling K., Herten M., Becker J. Photomed Laser Surg. All other parts of laser for the full warranty period. Sequentially, until the irradiation time reached 60 s per implant surface. Comparison of Er:YAG and Er:YSGG laser ablation of dental hard tissues Comparison of Er:YAG and Er:YSGG laser ablation of dental hard tissues Stock, Karl; Keller, Ulrich 1997-12-22 00:00:00 To compare ablation quality of Er:YAG and Er:YSGG laser the surface quality, crater shape, mass loss, and temperature development were determined using the same fiber transmission … Dental caries is determined by biological, be-havioral, psychosocial and environmental factors [2]. After formation of the biofilms, unattached cells were removed by washing with sterile phosphate-buffered saline (PBS), placed in a new sterile Eppendorf tube, and randomly allocated to the different treatment groups. Therefore, maintaininig the Sa of the implant after the decontamination method is an important goal that should be achieved for future re-osseointegration. The fiber tip used was an MZ8-6 mm (ZipTip) that was held perpendicular to the implant surface at a distance of 0.5 mm, using a custom-made mount and a scanning motion was used. In contrast to previous studies [18,19,24,39] that used Ti discs, we used commercially available implants to closely simulate the clinical situations regarding the complex implant topography. Souto R., Silva-Boghossian C.M., Colombo A.P.V. In the United States, the use of lasers on the gums was first approved by the Food and Drug Administration in the early 1990s, and use on hard tissue like teeth or the bone of the mandible gained approval in 1996. The honeycomb appearance was not observed in both groups II and III, however, a dense bacterial colonization covered the implant surface. Lee K., Yoon S.S. Pseudomonas aeruginosa biofilm, a programmed bacterial life for fitness. Al-Hashedi A.A., Laurenti M., Benhamou V., Tamimi F. Decontamination of titanium implants using physical methods. Adel S. Alagl, Marwa Madi, [...], and Zainab S. Al-Aql. Parlar A., Bosshardt D.D., Çetiner D., Schafroth D., Ünsal B., Haytaç C., Lang N.P. 1-3 The diversity of wavelengths, mode of energy delivery, and variety … Calcium solubility of dental enamel following Er, Cr:YSGG laser irradiation Calcium solubility of dental enamel following Er, Cr:YSGG laser irradiation Apel, Christian 2000-03-24 00:00:00 ABSTRACT Ever since the laser was introduced in dental medicine, there has been a constant discussion about its use in caries prevention. BIOLASE is the world’s leading innovator in dental lasers. Moley J.P., McGrath M.S., Granger J.F., Stoodley P., Dusane D.H. and S.B. The inhibitory effect of the diode laser (808 nm) on bacteria/LPS adherent to titanium oxide was observed spectrophotometrically through decreases in nitrous oxide/nitrite production which is an indicator of macrophage activation [24]. Stübinger S., Henke J., Donath K., Deppe H. Bone regeneration after peri-implant care with the CO. Marotti J., Geraldo-Martins V.R., Bello-Silva M.S., de Paula Eduardo C., Apel C., Gutknecht N. Influence of etching with erbium, chromium:yttrium-scandium-gallium-garnet laser on microleakage of class V restoration. and F.A.O. Due to the non-normal distribution, the Kruskal–Wallis test was used to assess difference among groups followed by pairwise comparisons among groups. The process of laser ablation produces an irregular, rough, and moist dental surface with exposed dentinal tubules, intact enamel rods, and no smear layer [ 5 ]. ; software, M.M. Peri-implantitis continues to pose a serious challenge to the success of osseointegration of dental implants. Laser photons energize atomized water spray and micro-droplets native to interstitial spaces in tissue, creating rapid expansion/micro-explosions in the water. Marti S., Soto S.M., Cisneros J., Pachón J., Pascual A., McQueary C., Actis L., Vilá J., Rodríguez-Baño J., Martínez-Martínez L., et al. The ePub format uses eBook readers, which have several "ease of reading" features The Er,Cr:YSGG laser is an effective alternative treatment modality for decontamination of dental implant surfaces without damaging the surface topography. Introduction: The objective of this study was to compare in vitro the resistance and type of failure in the debonding of lithium disilicate veneers with four different thicknesses using an erbium chromium yttrium-scandium gallium-garnet (Er, Cr: YSGG) laser.Methods: Sixty-eight bovine teeth were used to bond round lithium disilicate veneers with a 6-millimeter diameter and four … Strever et al. For operator’s safety, multi-wavelength protective eyewear was used and the cutting spray was directed toward the implant surface only. A multilayered A. baumannii biofilm was present on all implant surfaces: (D) 12,000×. Shahabi S, Ebrahimpour L, Walsh LJ. The aim of this in vitro study was to evaluate implant surface decontamination by means of Er,Cr:YSGG and diode lasers. 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